Abstract Details

Title Brain Temperature Elevations Parallel Unrestricted Water Movement And Increased Myelination In Temporal Lobe Epilepsy

Topic Epilepsy/Clinical Neurophysiology (EEG)

Presentation(s) S24 - Epilepsy/Clinical Neurophysiology (EEG): Clinical Epilepsy (3:54 PM-4:06 PM)

Poster/Presentation
Number 003

Background Focal brain temperature (T_CRE) elevations measured using volumetric magnetic resonance spectroscopic imaging (MRSI-t) may reflect neuroinflammation. FISO, a measure obtained using advanced diffusion MRI protocol and data processing tool (NODDI) is also thought to visualize neuroinflammation. However, both techniques have not been validated against each other and no studies to date evaluated whether T_CRE increases parallel NODDI-based microstructural pathology in epilepsy.

Objective To investigate group differences in and spatial overlap between brain temperature (T_CRE) and Neurite Orientation Dispersion and Density Imaging (NODDI) measures of microstructural injury in temporal lobe epilepsy (TLE).

Design/Methods 3T neuroimaging data were collected for 20 healthy controls (HCs; 22–52 YOA) and 20 TLE (22–71 YOA). Metabolite Imaging and Data Analysis System (MIDAS) and NODDI toolbox v1.01 were used to process the data. Voxelwise general linear models computed group differences in T_CRE and NODDI measures, with significance testing by the Threshold-Free Cluster Enhancement toolbox (p<0.05, corrected for multiple comparisons using family-wise error). The Fusion Independent Component Analysis (ICA) Toolbox tested the overlap between MRSI-t and NODDI. TLE participants’ data were flipped such that all had left (L) temporal onset.

Results Relative to HCs, T_CREwas elevated in TLEs’ temporal regions (L>R). In TLE, FISO was increased in left cerebellar and temporal regions, thalamus, and hippocampus. TLE participants had abnormally increased myelination in temporal and frontal cortices (L > R), and aberrant dendritic branching in the bilateral thalamus, R hippocampus, and L posterior frontal regions. T_CRE elevations paralleled myelination (r=0.68) and FISO (r=0.61) changes. The association between T_CRE and dendritic branching was weak (r=-0.30).

Conclusions Focal T_CRE in TLE corresponded to increased free water movement (i.e., edema) and myelination. Brain temperature elevations may reflect underlying microstructural injury, especially neuroinflammation and increased myelination that drives faster propagation of abnormal electrical signaling. Future analyses with larger sample sizes are necessary.

Authors/Disclosures
Jerzy P. Szaflarski, MD, PhD, FAAN (University of Alabama At Birmingham) PRESENTER	Dr. Szaflarski has received personal compensation in the range of $10,000-$49,999 for serving as a Consultant for UCB Pharma. Dr. Szaflarski has received personal compensation in the range of $10,000-$49,999 for serving as a Consultant for LivaNova Inc. Dr. Szaflarski has received personal compensation in the range of $500-$4,999 for serving as a Consultant for PureTech Health. Dr. Szaflarski has received personal compensation in the range of $5,000-$9,999 for serving as a Consultant for Gidley, Sarli & Marusak, LLP. Dr. Szaflarski has received personal compensation in the range of $5,000-$9,999 for serving as an Editor, Associate Editor, or Editorial Advisory Board Member for Elsevier. Dr. Szaflarski has received personal compensation in the range of $5,000-$9,999 for serving as an Expert Witness for Law Firm. Dr. Szaflarski has stock in AdCel Biopharma, LLC. Dr. Szaflarski has stock in iFovea.
Ayushe A. Sharma	Mrs. Sharma has nothing to disclose.
	No disclosure on file
	No disclosure on file