A retrospective observational cohort study was conducted linking the clinical database at the Southern Alberta HIV Clinic (SAC) with regional laboratory database to identify definitive cases of CNS infection from 1995-2019. CNS infection was categorized into OI (CMV, toxoplasmosis, PML and cryptococcal meningitis) and non-OI (EBV, coccidiomycosis, tuberculosis, neurosyphilis, HSV and VZV). Annual incidence rates (IR) of CNS infections per 1,000 person-years (PY) were calculated and collapsed into three time periods for OI and non-OI. Cox proportional hazards models were used to estimate hazard ratios(HR) and 95% confidence intervals ([,]) of risk factors for CNS infections in PWH.